| 出品公司: | Promega |
|---|---|
| 载体名称: | pBiT1.1-N[TK/LgBiT] |
| 载体又名: | pBiT1.1-N[TK/LgBiT]载体，pBiT1.1-N[TK/LgBiT]质粒，pBiT1.1-N[TK/LgBiT]载体质粒 |
| 英文名称: | pBiT1.1-N[TK/LgBiT], pBiT1.1-N[TK/LgBiT] Vector, pBiT1.1-N[TK/LgBiT] Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | HSV TK |
| 载体大小: | 3.8 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | |
| 产品目录号: | N201452 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
pBiT1.1-N[TK/LgBiT]
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pBiT1.1-N[TK/LgBiT]载体质粒基本信息
pBiT1.1-N[TK/LgBiT]质粒图谱载体图谱和pBiT1.1-N[TK/LgBiT]载体序列质粒序列多克隆位点信息
![pBiT1.1-N[TK/LgBiT]载体图谱](/uploads/载体图谱/pBiT1.1-N[TK-LgBiT]载体图谱.png)
![pBiT1.1-N[TK/LgBiT]多克隆位点](/uploads/载体图谱/pBiT1.1-N[TK-LgBiT]-MCS.png "pBiT1.1-N[TK/LgBiT]载体多克隆位点")
pBiT1.1-N[TK/LgBiT]质粒载体简介
pBiT1.1-N[TK/LgBiT]质粒序列载体序列
Snapgene载体序列下载: pBiT1.1-N[TK/LgBiT]序列 .gb格式载体序列下载: pBiT1.1-N[TK/LgBiT]载体序列
LOCUS pBiT1.1-N[TK/LgBiT] 3858 bp DNA circular SYN 19-JUL-2026
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3858)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jul 19, 2026 from SnapGene 8.1.0 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1135209600|
COMMENT VNTDBDATE|1135209600|
COMMENT VNTNAME|pBiT1.1-N [TK-LgBiT]|
COMMENT VNTAUTHORNAME|DotmaticsRD|
FEATURES Location/Qualifiers
promoter 28..779
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
regulatory 809..818
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
polyA_signal 1412..1533
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1952..2540)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(join(2740..3531,3532..3600))
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
polyA_signal 3705..3753
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 3767..3858
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
781 agcttggcaa tccggtactg ttggtaaagc caccatggtc ttcacactcg aagatttcgt
841 tggggactgg gaacagacag ccgcctacaa cctggaccaa gtccttgaac agggaggtgt
901 gtccagtttg ctgcagaatc tcgccgtgtc cgtaactccg atccaaagga ttgtccggag
961 cggtgaaaat gccctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgc
1021 cgaccaaatg gcccagatcg aagaggtgtt taaggtggtg taccctgtgg atgatcatca
1081 ctttaaggtg atcctgccct atggcacact ggtaatcgac ggggttacgc cgaacatgct
1141 gaactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt
1201 aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca cccccgacgg
1261 ctccatgctg ttccgagtaa ccatcaacag tgggagttcc ggtggtggcg ggagcggagg
1321 tggaggctcg agcggtggag ctcaggggaa ttcagtctaa gctagcagat cttctagagt
1381 cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
1441 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
1501 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
1561 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
1621 ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
1681 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
1741 gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc
1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
2701 ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt taccagtgct tgatcagtga
2761 ggcaccgatc tcagcgatct gcctatttcg ttcgtccata gtggcctgac tccccgtcgt
2821 gtagatcact acgattcgtg agggcttacc atcaggcccc agcgcagcaa tgatgccgcg
2881 agagccgcgt tcaccggccc ccgatttgtc agcaatgaac cagccagcag ggagggccga
2941 gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag tctatgagct gctgtcgtga
3001 tgctagagta agaagttcgc cagtgagtag tttccgaaga gttgtggcca ttgctactgg
3061 catcgtggta tcacgctcgt cgttcggtat ggcttcgttc aactctggtt cccagcggtc
3121 aagccgggtc acatgatcac ccatattatg aagaaatgca gtcagctcct tagggcctcc
3181 gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc atggtaatgg cagcactaca
3241 caattctctt accgtcatgc catccgtaag atgcttttcc gtgaccggcg agtactcaac
3301 caagtcgttt tgtgagtagt gtatacggcg accaagctgc tcttgcccgg cgtctatacg
3361 ggacaacacc gcgccacata gcagtacttt gaaagtgctc atcatcggga atcgttcttc
3421 ggggcggaaa gactcaagga tcttgccgct attgagatcc agttcgatat agcccactct
3481 tgcacccagt tgatcttcag catcttttac tttcaccagc gtttcggggt gtgcaaaaac
3541 aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat
3601 actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg
3661 ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat
3721 tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc
3781 atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag
3841 gtgccagaac atttctct
//
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